Regulatory
Part:BBa_K4307027
Designed by: Chi Zhang Group: iGEM22_Tsinghua (2022-09-30)
PmrC
This part is the promoter regulated by PmrB/A two-component system in E.coli. When PmrA (BBa_K4307026) is phosphorylated, it will combine with pmrC and start the gene expression. In Tsinghua 2022 project, it was used in affipmrABC (BBa_K4307042) system.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 372
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Characterization
The following figure demonstrates our successful construction.
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Categories
Parameters
None |